A point-scanning confocal laser scanning microscope built on a Nikon Ti2-E inverted stand, equipped with the A1R high-speed resonant scan head and a large 25 mm field of view. The setup at TU/e combines six excitation lasers, five detectors and two temperature-controlled stages, supporting both high-stability live-cell imaging and fast temperature-jump experiments. Hosted by the Soft Matter and Biological Physics group of dr. Janne-Mieke Meijer (Department of Applied Physics and Science Education).
Configuration
- Stand: Nikon Ti2-E inverted research microscope with Perfect Focus System (PFS)
- Scan head: A1R with 8 kHz resonant scanner
- Field of view: 25 mm
- Excitation lasers (6 lines): 405, 445, 488, 515, 561, 640 nm
- Detection: 5 detectors (4 confocal + transmitted light); brightfield mode with fast CCD camera
- Objectives: 10×, 40×, 60× water, 100× oil, 100× silicon oil
- Temperature control: Okolab stage incubator (0.02 °C stability) and VAHEAT stage heater for fast jumps (>5 °C/s)
- Software: Nikon NIS-Elements with 3D deconvolution and Denoise.ai
Located at
Soft Matter and Biological Physics lab, Flux building, TU/e campus.
Status
Operational. Owned and operated by the group of dr. Janne-Mieke Meijer; access by arrangement with the lab.
Questions or training? Ask the super-user
For general facility access or new project scoping, contact superresolution@tue.nl.
Vendor resources
Nikon A1 HD25 / A1R HD25 product page: microscope.healthcare.nikon.com.